Coding

Part:BBa_K2926057:Design

Designed by: Johanna Opgenoorth   Group: iGEM19_Bielefeld-CeBiTec   (2019-10-15)


Fusion protein of mating factor alpha, mCherry and pVIII with his-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To enable proper protein folding of every supdomain we designed a short glycine-serine linker sequence which was inserted in between the different subdomains.


Source

Mating factor alpha was obtained via oligosynthesis while mCherry was isolated from the iGEM part BBa_J06504 and the M13 major coat protein was excised from a helperphage plasmid purchased at NEB.

References